I have A260/A280 ratio of 4.3 from DNA extraction?

16 August 2023 1 9K Report

I have extracted DNA from synovial fluid from a knee and I have an A260/A280 ratio of 4.3 and ds DNA concentration of 1002ug/ml. Is my sample contaminated with something and does anyone have any idea of what this could possibly be? Thanks!

Murtadha Issa

The answer is a specialist in biological pollution, I am a geologist

Badges
Science topic

More Hollie Wilkisnon's questions See All

What speed should in centrifuge my samples to get bacterial cells?

I have some clinical samples of human synovial fluid and I am extracting the bacterial DNA. I was wondering it is possible to spin the samples to separate the bacterial cells from the human cells...

13 June 2023 496 4 View

How do I create a coding system in my qualitative action research project that creates a dialogue between image and text data sources?

Hello! I am currently sifting through a large amount of data including interview transcripts and photo-documentation of elementary student artwork and writing. As of right now, the coding system...

02 July 2019 2,302 4 View

Does CST Microwave Studio (v. 2017) allow users to label an isoline plot?

If yes, how does one do this? I'd like to export an isoline plot of the electric field generated around my model electrodes, but there doesn't appear to be any way to label the plot (e.g. with...

26 April 2019 1,855 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View