Hi Agustin,

what is the approximate concentration of the collagen solution you obtained? What is the pH of the solution, did you measured it? Above 5 g/l the solutions get very viscous, nearly gel-like (although it is not a gel since you need to increase pH and inonic strength to get allow physical crosslinking).

I suggest you take a small amount and try to dilute with acetic acid 0.01 M.

Best regards,

Marcus

Hi dear friend

I think it would better that you take a small amount and try to dilute with 8 M Urea, afterwrd,Concentration is achieved by spectrophotometer,and then It will be good to run the various concentrations of your protein to have control over reproducibility of the process.

Hope this helps,

Hi, thanks for your answers. The sample actually comes from bovine tendon. Not pig skin. I try to dissolve it in urea 9M, in acetic 0.5 M, in SDS 0.5% but it remains insoluble. I think it will be impossible to measure proteins or run a gel in these condition

Augustin, in which concentration range do you work?

Marcus

Hey!, 4mg/ml . The concentration was estimated by stove drying

ag

Hey. 4 mg/ml. The concentration of the sample was determined by oven drying.

ag