More information about your sample and what you are trying to analyse is required.
What are the compounds you are trying to analyse? Is it toluene or is toluene the solvent?
What is the sample currently dissolved in or has it already been dried down for dissolution in your HPLC solvent?
Are you doing normal or reverse phase HPLC?
For an HPLC analysis, the sample is usually dissolved in the same solvent as the initial solvent in your HPLC programme.
The solvents to be used also depends up on which detector you are going to use. For rest infromation I agree with Charles.
I agree with Drs. Charles and Balkrishna. Indeed, if you want to remove toluene, you can try to evaporate it under nitrogen gas stream. So you can substitute it with solvent to be used.
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