I don't know how to select microRNA to study with PPARgamma gene in diabetic patients, pls give me some advice
I want a program to use for LNA primer
05 June 2017 9,807 1 View
I have many reads from chicken growth hormone and I want to assemble these reads to form contig and to complete the whole sequence of chicken growth hormone in Iraqi native chickens.
05 June 2017 8,405 5 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
Suppose a material "A" has both tetrahedral and octahedral sites and we are doping another material "B" - usually an ion into it. How can we detect if the dopant has occupied the octahedral site...
17 July 2024 4,299 4 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello, From my (naive) understanding, there are several target genes of the canonical Wnt signaling pathway that serve as reliable/validated transcriptional readouts of pathway activity. These...
18 June 2024 4,699 0 View
Is there a protocol to establish diabetic conditions in pre-osteoblasts cell culture?
18 June 2024 9,306 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
It was gestalt psychologists that took issue with elementalism (Kohler 1929), that all perception/consciousness can be broken down into component parts. The expression ‘the whole is greater than...
16 June 2024 9,199 0 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View