I am wondering whether hFEN1 or hExo1 can cleave off 5'-bases instead of 3'-flap??

03 November 2019 0 2K Report

To be specific, can I use hFEN1 or hExo1 (structure-specific enzyme) or other human enzymes to only remove a part of 5' ssDNA chain (blue line) in dsDNA genome of a human nucleus?

Are there are in vitro data (figure) supporting this idea?

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