Hi!
If you plan to compare two ELISA techniques the results have to be differed no more than 5% (standart error). If this value is some more it means:
1. one kind of antibodies is not so well-specified.
2. you use too high or too low concentration of tested protein or used antibodies.
3. use any other ELISA kit which you used before for this protein for verification results.
And remember: no more than 5% between as two curves so in triple wells.
Good luck!
Dear Tatiana,
Where did you get 5% from? Do you have the reference?
Thank you,
Katrina
Dear Katrina,
It is generally accepted that imprecision should be lower than 10% for analytical determinations (Tecles et al. 2007. Analytical validation of commercially available methods for acute phase proteins quantification in pigs. Res Vet Sci 83:133–139). I have used this maximum limit of variation in all my validation studies. I hope it helps. Good luck!
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