I am using NIH3T3 cells from ATCC and found out that the cell culture contains diverse populations in terms of shape and expression of MHCI.

10 October 2012 0 8K Report

I am using this cell line to investigate tumorgenicity of some oncogenes as reported in the literature. Did anybody clone this cell line?

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Is anybody study gender bias for viral infection in the mice model?

I'm working with a mouse model and wondering how gender impacts on viral infection. Did anybody look into this? Thank you!

01 February 2015 8,167 1 View

Does anybody have suggestions on bacteria strains to culture unstable plasmids?

I am working with an unstable plasmid that mutations occurred during cloning in JM109. I'm wondering if any of you have the similar problem and how you work around this. Thanks for the help!

07 August 2014 288 4 View

Does anyone have experience in culturing rabbit cytotoxic lymphocytes (CTLs) in vitro?

We have a rabbit model system to develop vaccines. Because of the limitation of reagents for rabbit T cell culture, we have to optimize our in vitro culture conditions all the time. Nonspecific T...

09 October 2012 9,815 3 View

Is anybody working with tear samples?

I am detecting antibodies from tear samples. Although the antibody levels are signficantly lower than those from the corresponding serum samples, the noise of tear samples is much lower. I am...

09 October 2012 3,300 4 View

Does anybody know a good way (methods or technology) to quantify virus and monoclonal antibody?

We are studying virus-antibody binding and want to know how many antibody molecules can saturate a virus particle.

08 September 2012 3,651 14 View

Do you have unmet antibody needs in your research?

We might be able to help!

01 January 1970 9,404 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View