Hello Jiafen,

Yes, unstable, toxic or repetitive genes can be a serious pain. I suggest growing the plates and the liquid cultures at 30C instead of 37C and to use richer media (e.g., super medium) and not plain LB. As for strains with higher stability, I had a good experience with Stbl3 by Life Technologies, but even when using them I also used rich media and 30C.

Good luck!

Yehu

Thanks!

Also, one more strain called E. coli BLR strain is available from novagen. As both DE3 and non-DE3 variants.

Good Luck... :)

Thanks for the information!