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I'm working with a mouse model and wondering how gender impacts on viral infection. Did anybody look into this? Thank you!
01 February 2015 8,167 1 View
I am working with an unstable plasmid that mutations occurred during cloning in JM109. I'm wondering if any of you have the similar problem and how you work around this. Thanks for the help!
07 August 2014 288 4 View
We have a rabbit model system to develop vaccines. Because of the limitation of reagents for rabbit T cell culture, we have to optimize our in vitro culture conditions all the time. Nonspecific T...
09 October 2012 9,815 3 View
I am detecting antibodies from tear samples. Although the antibody levels are signficantly lower than those from the corresponding serum samples, the noise of tear samples is much lower. I am...
09 October 2012 3,300 4 View
I am using this cell line to investigate tumorgenicity of some oncogenes as reported in the literature. Did anybody clone this cell line?
09 October 2012 7,878 0 View
We are studying virus-antibody binding and want to know how many antibody molecules can saturate a virus particle.
08 September 2012 3,651 14 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...
14 July 2024 3,106 3 View
