Is anybody study gender bias for viral infection in the mice model?

I'm working with a mouse model and wondering how gender impacts on viral infection. Did anybody look into this? Thank you!

01 February 2015 8,100 1 View

Does anybody have suggestions on bacteria strains to culture unstable plasmids?

07 August 2014 238 4 View

Does anyone have experience in culturing rabbit cytotoxic lymphocytes (CTLs) in vitro?

We have a rabbit model system to develop vaccines. Because of the limitation of reagents for rabbit T cell culture, we have to optimize our in vitro culture conditions all the time. Nonspecific T...

09 October 2012 9,765 3 View

Is anybody working with tear samples?

I am detecting antibodies from tear samples. Although the antibody levels are signficantly lower than those from the corresponding serum samples, the noise of tear samples is much lower. I am...

09 October 2012 3,242 4 View

I am using NIH3T3 cells from ATCC and found out that the cell culture contains diverse populations in terms of shape and expression of MHCI.

I am using this cell line to investigate tumorgenicity of some oncogenes as reported in the literature. Did anybody clone this cell line?

09 October 2012 7,815 0 View

Does anybody know a good way (methods or technology) to quantify virus and monoclonal antibody?

We are studying virus-antibody binding and want to know how many antibody molecules can saturate a virus particle.

08 September 2012 3,607 14 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View