How to derive equations for a resonant buck converter with ZVS?

I am not understanding how each author is deriving the equations and the working principle. Individual authors are giving their own working principle explanation for different circuit topology....

12 February 2021 496 1 View

How to extract the yearly cash flow data (not time-series) from HomerPro in Excel? Also how to extract the monthly average electric production data?

I want to plot the monthly average electric production data and cash flow data in another software to get a more clear graph. For this, I need to extract the monthly or yearly data and not the...

11 February 2021 2,740 2 View

How commonly do you encounter Rummination Syndrome in your GI practice?

Do diaphragmatic breathing and speech therapy actually help? How often did you require multidisciplinary team approach?

17 January 2021 5,998 0 View

Can fibrosing cholestatic hepatitis in an immunocompromised patient be diagnosed without a liver biopsy?

provided that all the criteria for diagnosis has been fulfilled and alternative diagnosis are also ruled out. The patient has coagulopathy and transjugular biopsy wont be possible due to resource...

13 January 2021 8,935 4 View

What parameters should I use to measure human security in relation to environmental degradation in developing countries?

I wish to examine the impact of environmental degradation on human security in Bangladesh.

11 January 2021 3,282 6 View

What Research methodology I will use for Time series data?

I want to conduct a research which is based on time series data ( Suppose, Relation between GDP, Foreign direct investment, Import, Export). Previously I worked with panel dataset and I know the...

31 December 2020 1,207 6 View

Look for Book?

Can anybody give me the following writer book? Helen Deresky "International Management"

26 December 2020 5,109 5 View

What is the basic user power allocation mechanism for achieving maximum Spectral Efficiency(SE) in co-ordinated multi-cell OFDM wireless network?

Can someone help me to realize the basic concept of user power allocation for a wireless heterogenous OFDM network where there is a macro cell and inside it some femto cells. What is the basic...

01 December 2020 4,039 1 View

Do you think, citing a "preprint" as reference is logical in your future article?

Now a days many researchers are depositing their research paper draft as preprint in various web-based option, those are not peer reviewed yet or may submitted already or will be submitted in a...

29 November 2020 6,849 2 View

How can I apply Fine-tuning the deep CNN models feature extraction techniques in MATLAB for infrared-band (thermal) image data?

I want to apply Fine-tuning the deep CNN models like VGG-19, ResNet101, DenseNet, etc. using transfer learning with feature extraction techniques in MATLAB for infrared-band (thermal) image data....

12 November 2020 5,238 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View