Sayad Md Didarul Alam

2 Questions 5 Answers 0 Followers

Questions related from Sayad Md Didarul Alam

How to improve the yields of plasmid extraction?

My palsmid is around 9000 bp in pSectag2-MycHis-IRES-EGFP vector with ampicillin resistance and use DH-alpha E.coli.. It is highcopy plasmid. I tried both large and small volume of culture medium....

01 October 2018 9,561 4 View

I am trying to transfect S2 cell by pMT-Bip-GFP vector several time using cellfectine reagent. but did not succeed. Can anyone help me?

I am trying to transfect S2 cell by pMT-Bip-GFP vector. I tried several time using cellfectine reagent and also use Mirus Trans-IT reagents. but did not succeed. Can anyone help me?

13 October 2017 9,401 2 View