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Questions related from Sayad Md Didarul Alam
My palsmid is around 9000 bp in pSectag2-MycHis-IRES-EGFP vector with ampicillin resistance and use DH-alpha E.coli.. It is highcopy plasmid. I tried both large and small volume of culture medium....
01 October 2018 9,507 4 View
I am trying to transfect S2 cell by pMT-Bip-GFP vector. I tried several time using cellfectine reagent and also use Mirus Trans-IT reagents. but did not succeed. Can anyone help me?
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