Rohit Rajan

2 Questions 1 Answers 0 Followers

Questions related from Rohit Rajan

I am trying to resolve immune cytokines. Which is the right percentage of SDS resolving gel?

I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...

26 April 2024 9,827 0 View

Is there any alternate way to avoid perfusion using a perfusion buffer for isolation of liver cells for primary culture?

Currently, I am working on mouse or rat models' primary culture of liver cells (particularly hepatocytes). In most of the previous research, I found the protocol stressing the perfusion of the...

11 October 2023 3,705 0 View