Rohit Rajan

3 Questions 1 Answers 0 Followers

Questions related from Rohit Rajan

I am trying to resolve immune cytokines. Which is the right percentage of SDS resolving gel?

I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...

26 April 2024 9,831 0 View

Is there any alternate way to avoid perfusion using a perfusion buffer for isolation of liver cells for primary culture?

Currently, I am working on mouse or rat models' primary culture of liver cells (particularly hepatocytes). In most of the previous research, I found the protocol stressing the perfusion of the...

11 October 2023 3,713 0 View

Usually Retinoblastoma mutations manifest as eye cancer very early in children but mutations in p53 gene take significant time to manifest. Why?

Rb gene mutation causes Retinoblastoma that is prevalent in 2 year old children. Meanwhile p53 mutations result in variety of cancers which are phenotypically observable only after many years of...

30 April 2023 6,679 1 View