I am trying to regenerate plants from isolated protoplasts but after the first division the cells die. Any good protocols for protoplast culture?

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Majid Abdulhameed Ibrahim

Please, can you see these protocols. Thank you and good luck.

Adelle Roux

Thank you very much! This will definitely help. Do you know of any protocol specific for Nicotiana benthamiana or Nicotiana tabacum? I know protocols might vary between varieties.

Yuan-Yeu Yau

I have a protocol for Nicotiana tabacum. I regenerated plants from this protocol. Do you want a copy of this protocol?

Hi Yuan-Yeu Yau! I would really appreciate a copy, please. We have tabacum seeds (Samsun) which I can use.

Bikal Ghimire

you may go through this protocal

Cho, Y., Akhil Srivastava, & Nguyen, C. (2013). Laboratory Protocols in Fungal Biology, 395–401. https://doi.org/10.1007/978-1-4614-2356-0