I am trying to cross C. gattii R265 mating a with alpha. I have tried V8 and sporulation medium, but it did not work. Is there another medium or condition?
I mixed the matings, after that, I did the spot on the medium V8 ( pH 7.5 and 5.0) and sporulation medium (SD) and incubated at environment temperature, in the dark. I waited for more than one month and I could not observe the spores.
We did the same thing with C. neoformans and it worked.
Thank you!
Hi , some time temp and light period have strong effect of sporulation for some species.
ATCC uses Malt agar https://www.lgcstandards-atcc.org/Products/All/32608.aspx?geo_country=pl#culturemethod
I would also try Oat meal agar and a temp of 30C (parafilm seal the plates)
Oatmeal Agar
(OA)
Composition per liter:
Agar ............................................................................................ 12.0g
Oatmeal, rolled oats .................................................................... 10.0g
Glycerin .....................................................................................5.0mL
Lactic acid..................................................................................0.2mL
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