I am trying serum PCR for identifying bacterial genome, my question is why I am not receiving same results all the time working with same sample,?

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Asst.Prof.Muthanna Hadi Hussain

Because that the serum is not always having bacterial genome.

Alireza Mordadi

it seem be a personal technical issue when you use one sample.

check your work with another person as a observer.

Alemu Tekewe Mogus

You need to standardize and validate your protocols using appropriate controls.

Shubham Mathur

Dear Alemu Tekewe,

I am using same serum sample for establishing the parameter, if I had run the PCR 10 times with same parameters I got the result twice. and controls are standarize alreay