I am planning to use a vector. It have SV40_3_splice site and SV40_int site. What are the purposes of SV40_3_splice site and SV40_int site?

11 November 2016 0 6K Report

Hey Guys!

I am cloning my transgene in a vector. I observed that it have SV40_3_splice site and SV40_int site . I was wondering what are the functions of these site?

Please give a reference of any publication if possible.

Best,

ABS

Badges
Science topic

More Ankur B. Sharma's questions See All

Is fold change true representative of differential gene expression?

Hi Everyone, I have a simple query. When we estimate differential gene expression between two data sets or two conditions, we generally observed log(base 2)fold change. However, the fold change...

09 October 2018 2,776 4 View

Regarding fluorescence microscopy ?

Hi Guys, I am trying to make a stable cell line from NIH/3T3 by transfecting with my plasmid of interest. After transfection, while I was changing the medium, I applied a bit harsh pressure on...

09 October 2018 1,556 3 View

What are locus specific primers? How are they different from normal primers?

Hi everyone! I cam across term called "locus specific primer". I came across this term in 3C (chromatin conformation capture) method. I tried to google about it but could not understand much. Are...

06 July 2018 8,665 1 View

Do anyone know about an offline tool for RNA seq analysis?

Hello everyone I have to travel a lot in next few days but I don't want to waste time. Does anyone know any tool which can be used to perform RNA seq data analysis offline (from fastq files). I...

06 July 2018 4,575 4 View

Why it is important to take equal amount of total RNA for reverse transcription reaction (cDNA preparation)?

Hey everyone, I am working on liver tissue samples. I have FACS sorted 3 populations from same tissue and I want to study the gene expression between these populations using qPCR. One of the...

05 June 2018 6,435 11 View

What are the two population clouds in FACS data?

Dear all, I am trying to determine the DNA content in cell population by Hoechst 33342 DNA specific stain using flow cytometry analysis (BD FACS fortessa). I am observing two different clouds (...

04 May 2018 6,407 4 View

Karyotyping protocol for AML-12 cell lines?

Hi everyone, I was wondering if anyone have done the karyotyping for AML-12 cell lines (adult mouse liver cell lines)? I believe the protocol for karyotyping should be same like any other adherent...

31 December 2017 503 1 View

Regarding fluorescence microscopy ?

Hi Everyone, I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that...

10 November 2017 9,387 2 View

Culturing conditions for HepG2?

Hello All, I just started using HepG2 cell lines and I would like to know the culturing conditions. I have read through the ATCC guidelines for this cell line and they recommend EMEM+10% FBS....

09 October 2017 4,308 5 View

Is anyone using formaldehyde to fix cells and extract RNA from these cells later?

I want to sort cells using FACS, followed by fixation of sorted populations using formaldehyde or ethanol. I would like to extract total RNA from these sorted and fixed cells later. Has anyone...

07 August 2017 6,459 2 View

Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

A Question about Phd thesis?

Hello everyone What is your opinion about the introduction of an expert decision support system in which the rules are extracted from existing data without human intervention, instead of being...

31 July 2024 5,785 4 View

After a lot of feature engineering for CTR modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing?

After a lot of feature engineering for click-through rate modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing it?

29 July 2024 4,955 0 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View