Is fold change true representative of differential gene expression?

Hi Everyone, I have a simple query. When we estimate differential gene expression between two data sets or two conditions, we generally observed log(base 2)fold change. However, the fold change...

09 October 2018 2,694 4 View

Regarding fluorescence microscopy ?

Hi Guys, I am trying to make a stable cell line from NIH/3T3 by transfecting with my plasmid of interest. After transfection, while I was changing the medium, I applied a bit harsh pressure on...

09 October 2018 1,489 3 View

What are locus specific primers? How are they different from normal primers?

Hi everyone! I cam across term called "locus specific primer". I came across this term in 3C (chromatin conformation capture) method. I tried to google about it but could not understand much. Are...

06 July 2018 8,459 1 View

Do anyone know about an offline tool for RNA seq analysis?

Hello everyone I have to travel a lot in next few days but I don't want to waste time. Does anyone know any tool which can be used to perform RNA seq data analysis offline (from fastq files). I...

06 July 2018 4,462 4 View

Why it is important to take equal amount of total RNA for reverse transcription reaction (cDNA preparation)?

Hey everyone, I am working on liver tissue samples. I have FACS sorted 3 populations from same tissue and I want to study the gene expression between these populations using qPCR. One of the...

05 June 2018 6,359 11 View

What are the two population clouds in FACS data?

Dear all, I am trying to determine the DNA content in cell population by Hoechst 33342 DNA specific stain using flow cytometry analysis (BD FACS fortessa). I am observing two different clouds (...

04 May 2018 6,325 4 View

Karyotyping protocol for AML-12 cell lines?

Hi everyone, I was wondering if anyone have done the karyotyping for AML-12 cell lines (adult mouse liver cell lines)? I believe the protocol for karyotyping should be same like any other adherent...

31 December 2017 424 1 View

Regarding fluorescence microscopy ?

Hi Everyone, I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that...

10 November 2017 9,315 2 View

Culturing conditions for HepG2?

09 October 2017 4,234 5 View

Is anyone using formaldehyde to fix cells and extract RNA from these cells later?

07 August 2017 6,346 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View