I am a Ph.D. student in IISER, TVM. I want to do transfection in mouse embryonic stem cells using FuGENE. Please kindly provide with the detailed protocol.
I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...
26 June 2024 3,735 3 View
Dear Members of ResearchGate, I hope you are doing well. I have been retrofitting using the ASCE 41 2017 standard. In it, I have two basic doubts. 1. The target displacement as per clause...
13 June 2024 2,773 0 View
Hello Everyone, I hope you are doing well. What is the best book on seismic retrofitting reinforced concrete structures, preferably for Indian and US conditions (Recommendations please)? Thank...
30 May 2024 9,200 9 View
I am trying to understand how the expression of paralogues changed over time. I have real-time PCR data and gene tree. I want to reconstruct the estimated gene expression level at each node. I...
14 March 2024 8,131 0 View
Please can anyone suggest me how to write a book chapter in Elsevier?
21 February 2024 3,672 2 View
Hi Everyone, I want to know some of the best tools or platforms for planning research works (PhD or/and Master's research). Till now I have been using MS Excel to schedule my work, but it is not...
15 January 2024 6,985 3 View
dear researchers, I am interested in researching reading learning in elementary schools, can anyone help me with some books or articles?
15 January 2024 8,113 5 View
We are purifying the protein from recombinant DNA
05 January 2024 1,086 2 View
Can someone please help me by providing the values of electronic properties (bandgap,electron and hole density of states, dielectric permittivity and electron and hole mobilities) of Niobium...
02 January 2024 3,962 1 View
Hi everyone. I wish you all a Merry Christmas and a Happy New Year ahead! I hope you are doing well. I have a question. In Opensees, why reinforced concrete beam-column joint is assumed as a...
25 December 2023 3,540 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View