Tiyas Sengupta

5 Questions 1 Answers 0 Followers

Questions related from Tiyas Sengupta

Does 20% SDS gel melt during running at 120V?

My protein size is 17 kDa, and I cast a 20% gel. When the ladder started to resolve, we changed the voltage to 120V, but after some time, the ladder got stuck at one point, and the dye front...

24 July 2025 1,375 0 View

What should be the concentration of amphotericin in mouse embryonic stem cell culture?

I am culturing mouse embryonic stem cells and using DMEM, 10% FBS. I have added 2.5 ug/ml of amphotericin to the media (final concentration). But my cells were growing slowly and today onwards I...

05 December 2024 5,728 0 View

Can I use antibody used for ELISA in western blot?

I am planning to order an antibody, but for the commercially available antibody, it is written that the application is ELISA. So, if I want to order the same antibody can we use that?

20 August 2024 3,243 8 View

Is it okay to grow bacteria in 30 for cloning? What is the lowest mass of Vecor I can take for cloning? what should be the optimum insert ratio?

I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...

27 June 2024 4,006 3 View

I am looking for transfection protocol for mouse embryonic stem cells using FuGENE?

I am a Ph.D. student in IISER, TVM. I want to do transfection in mouse embryonic stem cells using FuGENE. Please kindly provide with the detailed protocol.

01 May 2024 9,159 0 View