Protocol for indirect elisa quantification of cytokines (IL-10, IFN-gamma) in serum/plasma?

31 December 2016 9,588 3 View

Hi, I am stuck in initial steps of cloning. Can anyone advise me how to clone 2.24kb long gene?

I am working on a gene which is 2.24kb long. I have already cloned its shorter form of 1700 bp. I have prepared cdna through Hek293 cell line, but when I put this gene's pcr I always get shorter...

31 December 2015 9,387 5 View

Can I use STATA9 software for linkage disequilibrium calculation?

Hi, I am working on two polymorphisms of a gene. I want to calculate if there is any linkage between them or not. I wonder if i can use STATA9 software for that? If anyone has information...

09 October 2015 462 0 View

Does UV radiation have any negative effect on PCR water? Exposing it in UV for 20 mins,can create any problems in PCR reaction?

Does exposing it in UV for 20 mins create any problems in a PCR reaction? By mistake I left PCR water under the laminar hood and switched on the UV for 15-20 mins. Now I have to perform a PCR...

02 March 2014 1,832 21 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View