Vertica Agnihotri

5 Questions 7 Answers 0 Followers

Questions related from Vertica Agnihotri

Protocol for indirect elisa quantification of cytokines (IL-10, IFN-gamma) in serum/plasma?

hii there, I would like to inform you that I'm working on IL-10 and IFN-gamma cytokines. I want to quantify these proteins levels in serum. I have tried using ELISA for the same but not getting...

01 January 2017 9,604 3 View

Hi, I am stuck in initial steps of cloning. Can anyone advise me how to clone 2.24kb long gene?

I am working on a gene which is 2.24kb long. I have already cloned its shorter form of 1700 bp. I have prepared cdna through Hek293 cell line, but when I put this gene's pcr I always get shorter...

01 January 2016 9,404 5 View

Can I use STATA9 software for linkage disequilibrium calculation?

Hi, I am working on two polymorphisms of a gene. I want to calculate if there is any linkage between them or not. I wonder if i can use STATA9 software for that? If anyone has information...

10 October 2015 481 0 View

I am looking for oral cancer cell lines. Can anyone provide proper information?

hii everyone,I have to initiate my work on oral cancer cell lines but unfortunately I am not able to get it any where in India. I have contacted NCCS (Pune) as well for the same concern but they...

10 October 2015 2,822 4 View

Does UV radiation have any negative effect on PCR water? Exposing it in UV for 20 mins,can create any problems in PCR reaction?

Does exposing it in UV for 20 mins create any problems in a PCR reaction? By mistake I left PCR water under the laminar hood and switched on the UV for 15-20 mins. Now I have to perform a PCR...

03 March 2014 1,854 21 View