I am looking for new effective adjuvant for mouse monoclonal. I have always used Freund. Any suggestion?

More Dominique Alfandari's questions See All

Hello, could you please give me details of the antibodies that can be used to perform quantitative immunofluorescence of AT1Rs in the mouse brain?

Hello, could you please give me precise details of the antibodies that can be used to perform quantitative immunofluorescence of AT1Rs in the mouse brain?

13 June 2024 3,591 1 View

Do cancer cells behave differently to healthy cells when exposed to hyperthermia ?

Dear colleagues, I'd like to find some references documenting the biological reactions of tumour versus normal tissue to heat-based ablations, in terms of cellular sensitivity/resistance and...

13 March 2024 4,814 2 View

(LCA - Ecoinvent) Where can I find the hypotheses behind the Ecoinvent EF 3.1 Caracterisation factors ?

Hello, I study the impact of activities on Oceans. 1. Ecoinvent is mistakingly considering the "infinite dilution" hypothese, forgetting that the dilution of pollution is usually done through its...

07 December 2023 8,172 1 View

Whistling 10 years ago: publication of the following was refused! a shame?

While «hair growth» is an extremely popular item. On February 12, 2013, there were 61,600,000 hits via Google and there were 70,400,000 hits on July 11, 2013? The interest grows on the web as the...

09 November 2023 9,327 1 View

Which tissue is closest to the prostate gland in terms of MRI features (e.g., relaxation times) and physiological characteristics ?

As the pig prostate (our animal model) is relatively difficult to access for an ablation procedure, we'd like to use a proxy organ.

03 September 2023 4,822 0 View

What is the best liquid-liquid extraction method to separate fatty alcohols for LCMS testing with or without derivatizing?

I have been trying to isolate a hydrophobic fatty acid compound from an aqueous extract with a surfactant. The fatty acid compound is Insoluble in water , soluble in ether, chloroform,...

30 August 2023 6,719 0 View

How to do an MRI image average and registration?

Hello, We have done an MRI acquisition of an animal brain using three times the same T1 sequence. However, we are now trying to average these three data sets and do a 3D reconstruction. This is...

22 March 2023 6,565 1 View

Why Rhodomonas salina does actively build aggregates?

Hi all, I recently observed that a Rhodomonas salina strain builds aggregates after only a few minutes. Does anyone have any idea why this is the case? Attached are two jpgs. Sorry for the poor...

22 February 2023 3,089 0 View

Behaviour Tests - Colour discrimination in birds of prey?

Dear Community, I’m searching for a researcher / research group who is working on behavioural tests – colour discrimination in birds of prey. I would be very grateful for advice and hints! Best,...

24 November 2022 9,176 1 View

Bird-window collision research in France?

Dear Community, I'm searching for bird-window collision research in France. Maybe somebody is aware of a research group which is looking into this topic? Best, Dominique

02 August 2022 9,288 2 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

How to measure the concentration of monoclonal antibodieswithout nanodrop ?

Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...

14 July 2024 3,106 3 View

Russell Linscott

Are you familiar with TiterMax?

http://www.titermax.com/

Dominique Alfandari

No I have not tried Titermax. I see the recommended rout is IM or SC is it effective IP as well? I am already immunizing with my antigen bound to agarose beads so the slow release and presentation is done.

Sorry, no direct personal experience. Just wanted to suggest some other options less commonly known. Here's another:

http://www.invivogen.com/quila

And we list a couple of others: https://www.linscottsdirectory.com/products?q=adjuvant

Hope that is helpful.