Using a mouse model, I will be collecting atrial and ventricular tissues at various ages. I will block the tissues in gelatin and generate 10um sections for immunohistochemistry.
Does anyone have suggestions for what is typically used in the field to identify cardiac dysfunction/ischemia immunohistologically?
I have seen reports that increased levels of Inosine and hypoxanthine occur following acute ischemia preceding increased troponin levels. Additionally, I have seen reports that proNGF increases in ateriols following myocardial infarct. I am new to this area of research and want to proceed properly.
Thank you everyone,
Using immunohistochemistry, Western blots, and gene expression analyses, also can be use a number of markers like ( troponins I and T, myoglobin, fibronectin),selected among those which are currently used in emergency departments to diagnose myocardial infarction .
You can go for immunohistochemical staining using antibodies to caspase3.
- Badges
- Science method