Extraction of proteins from adipose tissue is challenging because it has high lipid content and its protein amount is very low.
http://www.ncbi.nlm.nih.gov/pubmed/21565151
http://www.ncbi.nlm.nih.gov/pubmed/965844
http://diabetes.diabetesjournals.org/content/suppl/2012/12/12/db12-0584.DC1/DB120584SupplementaryData.pdf
http://www.ncbi.nlm.nih.gov/pubmed/21565151
http://www.ncbi.nlm.nih.gov/pubmed/965844
http://diabetes.diabetesjournals.org/content/suppl/2012/12/12/db12-0584.DC1/DB120584SupplementaryData.pdf
Here you have mine protoclol is working and our colleague is not:(
https://www.researchgate.net/post/How_to_prepare_the_sample_for_simple_SDS_PAGE_of_extracellular_matrix_derived_from_an_adipose_tissue
Keep fingers cross:)
There's a really good kit for that: http://www.inventbiotech.com/total-protein-adipose-tissues.php
The best and only commercial kit for protein extraction from Adipose Tissue is Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes. Here is the link: https://inventbiotech.com/products/minute-total-protein-extraction-kit-for-adipose-tissues-cultured-adipocytes
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