Thomas J Esparza

4 Questions 54 Answers 0 Followers

Questions related from Thomas J Esparza

What options are available for an overhead rotator that can handle 96-well deep blocks?

I am looking to mix immunoprecipitation reactions in a 96-well block and trying to find a rotator that can handle this requirement. Appreciate any insight beyond modifying an existing rotator.

14 August 2023 3,451 0 View

In search of: pH-dependent cleavable linkers for placement between protein domains. Any insights?

I am interesting in dumping protein cargo during endocytosis (pH 5-6) and interested to know if other researchers work with or have come across such a linker? Thanks for any advice or insights.

13 March 2023 7,200 0 View

DBCO-fluorophore performing at higher molecular weight in solution; any insight?

Working with DBCO-Cy7.5 to label particles. The molecular weight should be approximately 1100 daltons. By SEC the conjugate elutes as an appropriate low-molecular weight product. However, when...

12 June 2019 197 0 View

Iron oxide potential to quench fluorescent probes?

Curious if anyone has knowledge or experience with the potential quenching interaction between iron oxide and fluorescent probes? I am working with NIR probes and trying to determine if my issue...

12 June 2019 9,353 1 View