Thomas J Esparza

6 Questions 61 Answers 0 Followers

Questions related from Thomas J Esparza

What options are available for an overhead rotator that can handle 96-well deep blocks?

I am looking to mix immunoprecipitation reactions in a 96-well block and trying to find a rotator that can handle this requirement. Appreciate any insight beyond modifying an existing rotator.

14 August 2023 3,538 0 View

In search of: pH-dependent cleavable linkers for placement between protein domains. Any insights?

I am interesting in dumping protein cargo during endocytosis (pH 5-6) and interested to know if other researchers work with or have come across such a linker? Thanks for any advice or insights.

13 March 2023 7,268 0 View

Purification directly from Pichia buffered complex medium using metal affinity chromatography?

Does anyone have experience purifying protein directly from buffered complex media (recipe from EasySelect system) over a Ni-NTA column? I am trying to decide if I will need to run the media...

23 July 2020 1,722 5 View

DBCO-fluorophore performing at higher molecular weight in solution; any insight?

Working with DBCO-Cy7.5 to label particles. The molecular weight should be approximately 1100 daltons. By SEC the conjugate elutes as an appropriate low-molecular weight product. However, when...

12 June 2019 251 0 View

Iron oxide potential to quench fluorescent probes?

Curious if anyone has knowledge or experience with the potential quenching interaction between iron oxide and fluorescent probes? I am working with NIR probes and trying to determine if my issue...

12 June 2019 9,418 1 View

Can anyone help with plating rat primary hippocampal neurons?

I have recently started experimenting with establishing rat E18 hippocampal cultures. I have had mixed successes and failures and have learned a great deal along the way. One issue that I have...

05 April 2014 9,192 3 View