I am looking for a protein that is endogenously expressed in the nucleus of COS7 cells. Does anyone have recommendations?

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Provided that the shale conductivity termed here x increases with temperature, how could we compute it at reservoir temperature ?

one could use Waxman and Thomas (1974) work?

22 July 2024 6,637 0 View

Is there a difference between the curriculum and the style of the study?

A question about scientific methodology

13 July 2024 2,160 8 View

How Bpai (Branson dental operator posture assessment instrument is scored? How the total score is 194?

Hello Everyone, May I ask you to explain how the total score of BPAI is summed to 194? I know the scoring of modified BPAI well, but I need the original form. This is a link to the...

20 June 2024 7,284 0 View

How can biocatalysis be integrated into larger carbon conversion processes, such as biorefineries or waste valorization schemes?

biocatalysis can be integrated into larger carbon conversion processes, such as biorefineries or waste valorization schemes. The main question: HOW

18 May 2024 2,869 0 View

What are the environmental benefits of using biocatalysis for carbon conversion compared to traditional chemical methods?

The environmental benefits of using biocatalysis for carbon conversion compared to traditional chemical methods are large. What are they?

18 May 2024 3,620 0 View

Are there any recent developments in the discovery or engineering of novel biocatalysts for carbon conversion?

Developments in the discovery or engineering of novel biocatalysts for carbon conversion cannot be stopped. we need to know any recent deevelopment in this field.

18 May 2024 5,345 1 View

What types of carbon sources can be effectively converted using biocatalysis?

Types of carbon sources can be effectively converted using biocatalysis

12 May 2024 2,389 1 View

What are the strategies for optimizing biocatalysts for carbon conversion?

For carbon conversion , the use of biocatalysts must be optimized, so what are the strategies used for this purpose?

10 May 2024 7,839 1 View

How efficient are biocatalysts in converting carbon sources into valuable products compared to traditional chemical catalysts?

The biocatalysts can be more available than traditional catalysts? The quation is related to the sustainablity by conversion of carbon using biocatalysts.

04 May 2024 9,761 2 View

If possible, practical exercises on Coherens numerical modeling program?

I need training videos about the program to learn how to use it in order to complete some research and student projects.

15 February 2024 4,197 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View