I am looking for a method to stain spleen sections to identify germinal centers in mice. What stains, antibodies, or protocols do you use to verify the identity of a germinal center? Thank you!!
Use Ki-67 antibody (mouse monoclonal) and counter stain with an anti-mouse conjugated (fluorescent such as PE or FITC).
You could also stain for AICDA (activation-induced cytidine deaminase).
you should try anti-PNA or anti-CD95 in combination of anti-IgD or anti-CXCR4 to stain your GC structure
We have a system to treat water from an old industrial basin before discharging to the river. The system initially consisted of (in this order): a 3-stage bag filter, 3 granular activated carbon...
22 July 2019 9,298 3 View
Is it possible to mark shared first authors or joint senior authors of published articles on your research profile?
04 April 2019 8,967 6 View
My family and I would like to welcome any and all responses, advice, and any additional information that you would like to share. We would also like to thank you from the bottom of our hearts (in...
19 March 2019 2,640 5 View
I am running an agarose gel to check for RNA degradation, and I'm having trouble getting my sample to migrate out of the well. I've attached a copy of the picture showing the sample in the well....
19 February 2019 4,876 4 View
I'm looking for a method that is sensitive enough to measure dietary cellulose content of rat feed at 0.5% cellulose, and up to 1.5%. We have tried the ADF method and AOAC 991.43, the ADF was...
21 May 2018 2,237 1 View
Dear Colleagues, I am looking for the Malay version of the RSES, preferably the one Prof. Viren Swami (2011) studied in his chapter: Further Examination of the Psychometric Properties of a Malay...
06 November 2017 6,494 2 View
Does anyone have specific techniques or media to help the cell line increase its growth?
31 August 2016 8,770 1 View
09 June 2016 9,437 2 View
15 October 2015 5,571 18 View
30 January 2015 414 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
02 March 2021 3,060 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View