I am interseted for isolating microparticles/microvesicles from blood serum/plasma,Can anybody suggest me better kit for the same?

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Jekaterina Muhhina

We use ExoPrep for exosomes islotaion, for biofluids it gives a good result starting from 100 ul. Catalog code is HBM-EXP-B5(10 or 20), depends on amount, you need. Here you can find the product sheet http://www.exotest.eu/online_orders/image/data/inserts%20sheets/Exoprep.pdf

Gregory Barshtein

In addition to information presented by Jekaterina,

https://www.researchgate.net/publication/273466705_Methods_for_Extracellular_Vesicles_Isolation_in_a_Hospital_Setting

Article Methods for Extracellular Vesicles Isolation in a Hospital Setting

Peter Kubisz

Dear Parul,

in our experience , the most convenient method is flow cytometry.

Catalogs provided by colleagues are solid reference for starting.

Peter

Parul Sahu

Thank you Jekaterina Muhhina, Gregory Barshtein ,Peter Kubisz for your suggestions.

Dear Peter Kubisz,

Can you please explain it how I can isolate vesicles on flow cytometry. The bead attachment is the better option but I have no idea does it may affect the downstream processing.