I need pET30a-LIC-GFP vector to continue a study I had started on Epsilon 34 phage receptor binding protein, any help will be greatly appreciated. Thank you for your help in advance.
The pET30a-LIC-GFP vector is a plasmid that contains the gene for the green fluorescent protein (GFP) linked to a licencing element called the pLIC, which allows for efficient expression of the GFP protein in a wide range of host cells. This vector can be used to express the GFP protein in a variety of bacterial and eukaryotic cells, and it can be useful for studying the function of proteins in cells or for visualizing protein localization in cells.
If you need the pET30a-LIC-GFP vector for your research, there are several options you can consider:
Purchase the vector from a commercial supplier: There are many companies that sell the pET30a-LIC-GFP vector, and you should be able to find it through a quick online search. Some popular suppliers of this vector include Thermo Fisher Scientific, Sigma-Aldrich, and Addgene.
Obtain the vector from a colleague or a lab that already has it: If you know someone who has the pET30a-LIC-GFP vector, they may be able to provide you with a sample or point you in the direction of someone who can.
Order the vector from a repository: There are several repositories that maintain collections of plasmids, including the American Type Culture Collection (ATCC) and the German Collection of Microorganisms and Cell Cultures (DSMZ). These repositories may have the pET30a-LIC-GFP vector available for purchase or distribution.