The first band is my negative control.
Might be stimulant in cell media is causing bands to come
I have tried changing compliance current, delay. But I still couldn't figure out.
27 May 2024 512 0 View
Purification protocol for human PARP1 with good yield.
13 March 2024 9,746 3 View
Hi everyone, I am a PhD student exploring various genome sequencing approaches and NGS platforms before settling on one for my research. While searching WGS info, I found nothing helpful about...
10 February 2024 6,573 4 View
It is required for analyzing the runoff.
09 January 2024 5,626 5 View
Dear fellow connections, i am using elastomeric bearing plasticity element to model 2D bilinear isolators. But here, I also want to assign damping ratio of each isolator, does anyone know how can...
25 October 2023 1,330 0 View
I have used exponential damper in model but didnnot worked .At the end of run analysis,I got warning message.
28 September 2023 1,578 2 View
Historical building polygon shapefile
27 September 2023 8,273 1 View
Trying to convert dem data with ellipsoidal height to orthometric height.
23 May 2023 3,439 4 View
i am build the yagi anntenna .so i want improvement the antenna
20 May 2023 4,809 1 View
I am working on a 2D frame to study the response of Base isolated structure. I did modal analysis analytically in MATLAB and tried comparing with modal analysis output of ETABS. The modal time...
30 April 2023 4,937 2 View
I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...
04 June 2024 6,924 0 View
I have quantified the lysate by bradford method and loaded 25ug of lysate for SDS PAGE and proceeded for western blot. However, upon detection I see a variation in loading control i.e., GAPDH. I...
15 May 2024 3,433 6 View
There are 33 items adding up to an overall value for qol score. How to present the results of two-step cluster analysis in the methodology for differentiating cases and controls group? Should any...
14 May 2024 7,716 2 View
i need some help setting up my culture plate having negative controls,I am going to infect HEK293T cells in a 6-well plate... to one well I will add my lentivirus particles(packaged already), and...
15 April 2024 7,218 5 View
I am optimizing an indirect ELISA for a flavivirus. All my samples are suspected cases. I have the mean and standard deviations of positive controls. When I use a positive standard deviation,...
14 April 2024 1,276 2 View
Why did someone average the control? When selecting your calibrator sample you can select just one of the ‘controls’ that way only 1 sample in the experiment will have a 2^-ddCT of 1.I see a lot...
06 April 2024 3,866 0 View
Hello,Goal is finding concentration of PS20, prepared std curve concentrations of , 25,50,100,150 and 200 ug/mL , after methylene chloride extraction of PS20, and reading at UV spectromete at...
13 March 2024 8,332 0 View
This is a case-control study where I have assessed near-work performance (having 3 dependent continuous variables) in the two groups (one with vision disorder and normal controls). I want to...
13 March 2024 9,765 3 View
I am making tests for diagnostic PCRs. At the moment I am trying to standardize a pcr reaction for a circa 180 bp product. I did not get positive bands so far. Interestingly, I get what appears to...
26 February 2024 2,820 3 View
Gubernaculum gives rise to smooth and striated muscles around processus vaginalis. Descent of a testis is succeeded by propelling the testis through processus vaginalis. After propelling the...
23 February 2024 5,891 0 View
