Roshan Ghimire

2 Questions 3 Answers 0 Followers

Questions related from Roshan Ghimire

I have been facing problem of cell layer coming off during virus titer especially while incubating cells with virus for 1 hour for attachments?

I am been using VEROTMPRSS2 cells and incubating cells with 200ul of virus dilution for one hour. Cell layers are coming off after 30 min incubation. What might be the best solution to keep cell...

08 November 2023 9,779 2 View

How to see phosphorylation of p-IRF3 protein in mouse macrophages cell line? I used HMW poly I:C(10ug/ml) to stimulate the cells but no results?

I collected samples at 30min,1 hrs,2 hrs,6hrs,,12hrs and 24 hrs. But still couldn't detect phosphorylation. If anyone has done it, could you please suggest me better stimulation and collection time.

29 September 2023 5,029 2 View