Hello,Goal is finding concentration of PS20, prepared std curve concentrations of , 25,50,100,150 and 200 ug/mL , after methylene chloride extraction of PS20, and reading at UV spectromete at 320. Taking the same cuvette that has STD 2 , which is 50ug/mL, reading it as unknown to confirm the integrity of the system against the new curve that I generated, I get 44ug//mL not 50ug/mL . Help me please. I also made duplicate controls at 50ug/mL . Rep 1 reads 40.5 ug/mL and rep 2 reads 49.9 ug/mL, Both controls prepared identical, what am I doing wrong ?
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