I am making a site directed mutation in a gene using PCR.  The two PCR pieces are then purified using a kit.  PCR shows that each piece is the correct size (200bp and 1100bp) and a single band.  After running the assembly PCR step, I end up with a smear on my gel.  I have changed temperatures and concentrations of the primers and PCR pieces.

Any suggestions on things to check/change? 

Thanks in advance,

Derek

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