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Questions related from Derek Englert
I am making a site directed mutation in a gene using PCR. The two PCR pieces are then purified using a kit. PCR shows that each piece is the correct size (200bp and 1100bp) and a single band....
07 July 2016 9,489 9 View
I have a PCR product made of 2 pieces. I am doing site direct mutagenesis of an enzyme. I use PCR to create the 2 parts. I purify the 2 parts using a cleanup kit. I then run PCR a second time...
02 February 2016 2,106 4 View
In the near future I will need DNA sequencing to confirm mutations. Could someone point me in the direction of a cost effective company? The gene itself is around 1300 base pairs. The entire...
08 August 2015 7,241 4 View
