I want to dissolve Collagen Type 1 (rat tail) 100 milligms , 3.77 MG/ML to the final 25X in PBS?

I have got this RAT TAIL Collegen type 1 from BD bio-sciences and wanted to dissolve it to final 25X conc. in PBS. I just want it for coating the plate , working with primary hepatocytes. Any...

05 June 2018 5,912 1 View

How to process brain tissue for confocal microscopy?

I have fresh frozen and formalin-fixed brain tissue and I am planning to do confocal to access mitochondrial morphology what will be the best route to go? 1- Frozen or 2- Formalin-fixed Also, I...

01 February 2018 264 3 View

The Western Blot stopped giving any bands, looking for constructive answers?

I have been doing western blots on human brain samples since last 3 to 4 months regularly and the bands were coming pretty good. But all of a sudden I cannot detect any bands, even for Actin.I...

11 December 2017 5,398 15 View

Whats the highest resolution image of eukaryotic organism obtained till date?

06 July 2016 9,526 0 View

If we innoculate 5 strains together in broth medium what will happen (Detailed question below)?

What will happen if we innoculate five strains of Bacillus subtilis, B. cereus, B. amyloliquefaciens, B. pumilus and B. sphareicus together in a broth medium? Whether they all flourish well or...

03 April 2016 9,838 2 View

How can one determine the concentration of Kinesin - GFT ?

Suppose that you had run out of Coomassie blue reagent to stain protein in the SDS-PAGE gel, but still needed to determine how much kinesin - GFP you had in your purified sample. You do, however,...

08 September 2014 7,425 4 View

Can someone help with a article based on drug delivery, nanoparticle platform in atherosclerosis with relevant clinical application?

Any paper related to clinical trails or related to research based on nano-particle and related to atherosclerosis will be helpful. Any suggestion will be highly appreciated?

06 July 2014 6,282 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View