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Questions related from Amar Kumar
I have got this RAT TAIL Collegen type 1 from BD bio-sciences and wanted to dissolve it to final 25X conc. in PBS. I just want it for coating the plate , working with primary hepatocytes. Any...
06 June 2018 5,951 1 View
I have fresh frozen and formalin-fixed brain tissue and I am planning to do confocal to access mitochondrial morphology what will be the best route to go? 1- Frozen or 2- Formalin-fixed Also, I...
02 February 2018 302 3 View
I have been doing western blots on human brain samples since last 3 to 4 months regularly and the bands were coming pretty good. But all of a sudden I cannot detect any bands, even for Actin.I...
12 December 2017 5,437 15 View
I am getting a non - specific band, it suppose to be for the protein IP3R at around ~240 but inspite of that a really prominent intensity band obtained at around ~ 135 - ~150 range. The band is...
10 October 2017 3,029 4 View
Cryo electron, eukaryotic, high resolution image
07 July 2016 9,569 0 View
What will happen if we innoculate five strains of Bacillus subtilis, B. cereus, B. amyloliquefaciens, B. pumilus and B. sphareicus together in a broth medium? Whether they all flourish well or...
04 April 2016 9,931 2 View
Suppose that you had run out of Coomassie blue reagent to stain protein in the SDS-PAGE gel, but still needed to determine how much kinesin - GFP you had in your purified sample. You do, however,...
09 September 2014 7,466 4 View
Any paper related to clinical trails or related to research based on nano-particle and related to atherosclerosis will be helpful. Any suggestion will be highly appreciated?
07 July 2014 6,319 3 View
