How to process brain tissue for confocal microscopy?

I have fresh frozen and formalin-fixed brain tissue and I am planning to do confocal to access mitochondrial morphology what will be the best route to go? 1- Frozen or 2- Formalin-fixed Also, I...

01 February 2018 264 3 View

The Western Blot stopped giving any bands, looking for constructive answers?

I have been doing western blots on human brain samples since last 3 to 4 months regularly and the bands were coming pretty good. But all of a sudden I cannot detect any bands, even for Actin.I...

11 December 2017 5,398 15 View

I am getting a non -specific band on my western blot ~ 135 - ~150 range?

I am getting a non - specific band, it suppose to be for the protein IP3R at around ~240 but inspite of that a really prominent intensity band obtained at around ~ 135 - ~150 range. The band is...

09 October 2017 2,986 4 View

Whats the highest resolution image of eukaryotic organism obtained till date?

06 July 2016 9,526 0 View

If we innoculate 5 strains together in broth medium what will happen (Detailed question below)?

What will happen if we innoculate five strains of Bacillus subtilis, B. cereus, B. amyloliquefaciens, B. pumilus and B. sphareicus together in a broth medium? Whether they all flourish well or...

03 April 2016 9,838 2 View

How can one determine the concentration of Kinesin - GFT ?

Suppose that you had run out of Coomassie blue reagent to stain protein in the SDS-PAGE gel, but still needed to determine how much kinesin - GFP you had in your purified sample. You do, however,...

08 September 2014 7,425 4 View

Can someone help with a article based on drug delivery, nanoparticle platform in atherosclerosis with relevant clinical application?

Any paper related to clinical trails or related to research based on nano-particle and related to atherosclerosis will be helpful. Any suggestion will be highly appreciated?

06 July 2014 6,282 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View