I have got this RAT TAIL Collegen type 1 from BD bio-sciences and wanted to dissolve it to final 25X conc. in PBS. I just want it for coating the plate , working with primary hepatocytes.
Any help will be appreciated.
Thank You!
Thank you Hiba Riyadh Jameel
I have fresh frozen and formalin-fixed brain tissue and I am planning to do confocal to access mitochondrial morphology what will be the best route to go? 1- Frozen or 2- Formalin-fixed Also, I...
01 February 2018 323 3 View
I have been doing western blots on human brain samples since last 3 to 4 months regularly and the bands were coming pretty good. But all of a sudden I cannot detect any bands, even for Actin.I...
11 December 2017 5,456 15 View
I am getting a non - specific band, it suppose to be for the protein IP3R at around ~240 but inspite of that a really prominent intensity band obtained at around ~ 135 - ~150 range. The band is...
09 October 2017 3,052 4 View
Cryo electron, eukaryotic, high resolution image
06 July 2016 9,586 0 View
What will happen if we innoculate five strains of Bacillus subtilis, B. cereus, B. amyloliquefaciens, B. pumilus and B. sphareicus together in a broth medium? Whether they all flourish well or...
03 April 2016 9,976 2 View
Suppose that you had run out of Coomassie blue reagent to stain protein in the SDS-PAGE gel, but still needed to determine how much kinesin - GFP you had in your purified sample. You do, however,...
08 September 2014 7,488 4 View
Any paper related to clinical trails or related to research based on nano-particle and related to atherosclerosis will be helpful. Any suggestion will be highly appreciated?
06 July 2014 6,338 3 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View