How to process brain tissue for confocal microscopy?

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I want to dissolve Collagen Type 1 (rat tail) 100 milligms , 3.77 MG/ML to the final 25X in PBS?

I have got this RAT TAIL Collegen type 1 from BD bio-sciences and wanted to dissolve it to final 25X conc. in PBS. I just want it for coating the plate , working with primary hepatocytes. Any...

05 June 2018 5,970 1 View

The Western Blot stopped giving any bands, looking for constructive answers?

I have been doing western blots on human brain samples since last 3 to 4 months regularly and the bands were coming pretty good. But all of a sudden I cannot detect any bands, even for Actin.I...

11 December 2017 5,456 15 View

I am getting a non -specific band on my western blot ~ 135 - ~150 range?

I am getting a non - specific band, it suppose to be for the protein IP3R at around ~240 but inspite of that a really prominent intensity band obtained at around ~ 135 - ~150 range. The band is...

09 October 2017 3,052 4 View

Whats the highest resolution image of eukaryotic organism obtained till date?

Cryo electron, eukaryotic, high resolution image

06 July 2016 9,586 0 View

If we innoculate 5 strains together in broth medium what will happen (Detailed question below)?

What will happen if we innoculate five strains of Bacillus subtilis, B. cereus, B. amyloliquefaciens, B. pumilus and B. sphareicus together in a broth medium? Whether they all flourish well or...

03 April 2016 9,976 2 View

How can one determine the concentration of Kinesin - GFT ?

Suppose that you had run out of Coomassie blue reagent to stain protein in the SDS-PAGE gel, but still needed to determine how much kinesin - GFP you had in your purified sample. You do, however,...

08 September 2014 7,488 4 View

Can someone help with a article based on drug delivery, nanoparticle platform in atherosclerosis with relevant clinical application?

Any paper related to clinical trails or related to research based on nano-particle and related to atherosclerosis will be helpful. Any suggestion will be highly appreciated?

06 July 2014 6,338 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View