How to do the calculation for super oxide dimutase assay and how to calculate the R squared value in regression equation for catalase assay?

i have the enzymatic antioxidant assay by following the method of Misra and Fridovich, 1972 for super oxide dimutase and Luck, 1974 for catalase assay can any help me how to the calculation for...

11 February 2021 2,241 3 View

The peptide synthesized with CTC resin have mass more (+68 Da) than the desired mass of the synthesized peptide. What could be the reason?

I am synthesizing 7 amino acid long peptide sequence with CTC resin, 50oC coupling for 10 min. On cleavage (TFA/TIPS/Water 95:2.5:2.5) I got peptide with good yield. However, It has 68 Da more...

30 January 2021 8,483 3 View

How can we draw the graph of a function of complex variables involving two variables in Matlab?

Suppose the function is $f(z_1, z_2) = z_1^2+z_2^2$, where $z_1, z_2 \in \mathbb{C}$. How to implement the graph in Matlab in an given interval. Can anyone help?

11 January 2021 1,018 2 View

For VANET related issue /problems like congestion , MATLAB or NS2/NS3?? Which will be more suitable for implementation??

I have gone though few papers related to this problem. Researchers used NS2.35/2.38. and MATLAB. Also. My particular question is regarding congestion control . Which will be more suitable to use ?

22 December 2020 8,197 10 View

Physical significance of Gap between optical and acosutic branches in Phonon Dispersion?

Hello Everyone, Recently I am doing some Phonon branchesbased studies. For some materials the gap between the optical and acoustic branch is small, for some it's large and for some there is no...

04 November 2020 3,987 2 View

Can I perform islet isolation after storing the excised mouse pancreas for 3 months and what is the protocol for this process?

I have sacrificed treated BALB/C mice and collected the pancreas and stored in -20 degree centigrade for 3 months. Now I want to isolate the islets to study the release of different cytokines from...

08 October 2020 7,299 6 View

2 subsequent saccade and fixation duration after filtering 'unclassified' and 'eye not found' data in Eye tracking study?

My study is to use only fixation and saccade data. But after removing :unclassified' and 'eye not found' data. I come to know there is 2 back to back fixation duration or saccade what I need to...

28 September 2020 9,919 3 View

How informal learning can impact the learning culture of the organization?

Need to know specific parameters which impact the learning culture variables.

23 September 2020 233 4 View

How can i simulate dual-X second generation current conveyor using CMOS?

24 August 2020 8,067 4 View

In quantitative study, for calculating sample size, can the precision be taken 0.08?

In health related studies, can the level of precision be taken as 0.07 or 0.08 while calculating sample size?

19 July 2020 3,230 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View