I've only used the bionalyzer, but as far as I know, both the bioanalyzer and the tape station are essentially the same technology, with differences in throughput.

Nevertheless, what I have learned from the bioanalyzer is that been able to see 2 good peaks for the rRNA does not mean they are not degraded. What the RIN is telling you, is the ratio between both peaks. In conclusion, you can see nice bands but still have a bad RIN value.

Thus, your RIN value tells that your RNA is degraded. You should check for your RNA purification protocol, because you might be introducing RNAses at some point. Plus, I would use a purification kit specifically designed for plant isolation.

Additionally, and having no information about sample conservation or purification in your protocol, I have found that sample conservation is of paramount importance. As an example, for corneal RNAs from slaughtered pigs, after RNA purification RIN values are pretty bad. Given that my conservation and purification protocol was already optimized when I did this, I concluded that RNA must have been degraded after pigs were slaughtered and before I put the samples in a conservation reagent (RNAprotect, Qiagen). It was only 4-5 hours after slaughter that I took my samples.

So: check for the time between "plant killing" and purification, sample conservation, and RNA purification procedure.

Hi shweta, if the sample concentration is  high dont load directly normalise all the samples in same ratio basing on qubit reading or nanodrop.