Our -80 degree freezer is not working, and I have no other option to store the tissue at -20 degree for RNA extraction. Is it possible?
It is possible with a solution made to store tissue:
http://tools.thermofisher.com/content/sfs/manuals/cms_056069.pdf
It is ok, but not for a long time.
I agree with katie, you may store your samples directly in RNAlater at -20 or -80 degree refrigerator for indefinite time. But if you have not stored you sample in this reagent better you prepare cDNA as soon as possible.
Please let me know the freely available tools for analyzing reference gene expression stability in Real time PCR?
31 December 2017 6,401 4 View
10 November 2016 9,250 2 View
I've extracted RNA from plant tissues, and want to store it at -20 degree. Is it possible?
01 January 1970 1,149 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
02 March 2021 3,060 3 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View