Can I store plant tissue samples at -20 degree for RNA extraction after snap-frozen in liquid nitrogen?

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Can I store RNA at -20 degree, as our -80 deep freezer is out of order. If yes, then how long RNA can be stored at -20 degree?

I've extracted RNA from plant tissues, and want to store it at -20 degree. Is it possible?

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Katie A Burnette

It is possible with a solution made to store tissue:

http://tools.thermofisher.com/content/sfs/manuals/cms_056069.pdf

Huiquan Zheng

It is ok, but not for a long time.

Shaifali Pal

I agree with katie, you may store your samples directly in RNAlater at -20 or -80 degree refrigerator for indefinite time. But if you have not stored you sample in this reagent better you prepare cDNA as soon as possible.