Iker Garcia

2 Questions 39 Answers 0 Followers

Questions related from Iker Garcia

What is the method of choice for RT-qPCR normalization (relative gene expression analysis)?

I want to calculate the % increase in gene expression of a particular transcript after treatment with antisense oligonucleotides to skip a mutated exon. 1) I can not use reference genes: no...

10 October 2015 2,107 12 View

Any experience on the use of commercial CRISPR/Cas9 systems (no plasmid needed)? Which would you recommend?

I am about to start a new project on gene editing (deletion of specific exons). We were planing to use a plasmid from addgene, but we are short in staff and time; thus, we are considering the use...

01 January 1970 5,885 5 View