What causes the white coloration in shelf fungi?

During late June of 2020, I had picked some relatively fresh Schizophyllum commune. By the time I got around to examining them, they had become dried and shriveled. I placed the point of...

02 December 2020 4,193 3 View

Is there a proper citation for the human genome assemblies?

I am currently writing up results from a study that utilized the hg19 [Genome Reference Consortium Human Build 37 (GRCh37)] for analysis and I have not been able to identify a way to cite this...

24 November 2020 9,960 2 View

Professional Engineer's Code of Ethics: What & How To Assure Compliance?

Historically, the subject question has been met mainly with "After The Fact" assertions. Yet when one learns of the infrastructure failures . . .bridges, tunnels, pipelines, highways. . . it...

11 November 2020 3,574 3 View

Parsing error in autodock vina?

I'm trying to use autodock vina, and I received the following error for the ligand file when running the configuration: "Parse error on line 26 in file "15.pdbqt": Unknown or inappropriate...

21 October 2020 7,245 1 View

How do we use adjusted prevalence rate (aPR) in a meta-analysis?

Hi, I am doing a meta-analysis using Review manager determining association between increase in pollutant concentration and the risk of a particular disease. Majority of outcomes are reported as...

20 October 2020 10,012 3 View

How can I calculate prevalence ratio on Stata?

Hello, Our study aimed to estimate the stress prevalence among health professionals who works on pre-hospital services. The dependent variable was the presence or absence of stress (dichotomic...

16 August 2020 9,595 5 View

Q1. What changes have been observed in the professional lives of our CE graduates ? Q2. What CE academic programs have developed?

In June 1995, the culmination of some 3 years or so of preparation, led by professor Dr. Jim Yao, Texas A&M, resulted in an "ASCE Education Conference" in Denver, CO. At the end of that...

12 August 2020 1,069 3 View

How can I delete a document and replace it with proper text?

I have added a new document to my documents but found it was the wrong file. How do I delete the current document so I can replace it with correct one.?

03 August 2020 292 1 View

No title

16 June 2020 2,168 1 View

Native English speakers and/or PIV intermediates: Could you check the wording of my PIV tool...?

Hello everyone, I have made a free PIV tool for Matlab in English, but I am not a native speaker. I would like to make the tool as clear as possible because it is used quite often. Could someone...

09 June 2020 5,449 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View