I accidentally included the PAM sequence at the 3' end of a CRISPR guide RNA. Any chance it will work (knockout)?

The guide RNA is 5'-17n's-GGG-3'. There is no other PAM adjacent to the 3' end that is not part of the gRNA. This is intended to be a simple double-stranded cut to knock out the gene.

21 February 2021 553 1 View