Yes, it's a good idea. We aren't certain we've found all the protein-coding genes yet, especially ones that come from short coding sequences or are made entirely of short exons.

Starting with the Y chromosome isn't that smart, as the Y chromosome is thought to have a far lower density of expressed genes than the other chromosomes. However, that in itself makes it interesting.

The biggest problem I would think is finding the right tissue source. Where would you expect genes on the Y chromosome to be expressed?

Well, I think human seminal plasma is a good source for looking for Y chromosme proteins. No? It is non-invasive etc.

Good idia

but as you told NCBI have 420 genes and expasy had 50 proteins. It is not essential that all gene translate in a system, it depends on requirement of system. protein translate by signal trnasduction, so we nwver got all gene products.

Its a good idea to map the proteins to chromosome. Most probably the conceptual translation method will be the theoritical and collection of the expressed gene products at various stages of life ie, infant, baby, childhood, adolsence, adult, and old might bring some insight to it. Furher, the tissue source may also be concerned with developing time, exactly where the genes are expressing. I also think that there are some environmental stimulants responsible for expression of certain genes.

It is always a good idea to "Divide and Conquer", given the dynamic range and complexity of human proteome. But I think Simple proteomics searches would not reveal much and we should try adavanced proteogenomic approaches for better insights, alongwith orthogonal techniques like RNA-Seq etc.

It is very good idea and hope will work

Hope we can find solution for many diseases if it works.............