I want to co-culture bone marrow-derived MSc and M2 macrophage for 14 days.
1. It is necessary to supply IL-4 and IL-10 for maintaining the plasticity of M2 macrophage?
2. Or the MSC released growth factor is sufficient for maintaining the plasticity of M2 macrophage
Dear Subhadeep!
Please You look at the article:
Article Macrophage type modulates osteogenic differentiation of adip...
Direct co-culture of macrophages and MSCs
Direct co-culture of macrophages and MSCs at different ratios
Indirect co-culture of macrophages and MSCs
Indirect co-culture of macrophages and MSCs by a transwell system
MSCs were known to facilitate monocyte to macrophage transition. They tend to skew naïve macrophages to a pro-inflammatory M1 polarized state.
However, but interestingly, when co-cultured with M1 macrophages, they were shown to attenuate M1 phenotype and further enhance M2 functional states indicating a M1 to M2 shift upon MSC priming. Moreover, MSC-M2 macrophage co-culture was shown to either maintain or enhance the M2 phenotype further indicating maintenance of the M2 phenotype.
So, to your question, I believe that you can stick to your point 2 "MSC released growth factor is sufficient for maintaining the plasticity of M2 macrophage".
If I were you, I would test this in my pair of hands.
As such, no change of media is required…point to be noted that if the media color turned yellowish, then keep changing the media every after 3 days.
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