HPV universal primer sequence?

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I am carrying out an experiment on Trichoderma. How can I measure OD of the fungal growth after 7 days?

I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....

11 July 2024 6,251 1 View

What does Habitat Occupancy mean? How to calculate Habitat Occupancy for a small animal species?

I want to assess the habitat occupancy of a small species in a habitat. What should I collect as field data to assess its habitat occupancy?

06 February 2024 9,039 4 View

Why has development cooperation been less productive in less developed countries?

Is it because of the weak governance of the recipient countries or the hidden interests of the development partners that influence the policies?

28 December 2023 377 3 View

How can I access it to publish my article?

I want to publish my academic article in this journal. How is it possible?

26 December 2023 421 4 View

What is the specific impact of microsatellite marker analysis on unraveling the genetic diversity and population structure of sheep in Nepal?

What is the specific impact of microsatellite marker analysis on unraveling the genetic diversity and population structure of sheep in Nepal, and how does this information contribute to the...

26 November 2023 3,797 0 View

Why Big Data are to be secured? Is it any threat to National Security?

Data Security Big Data

14 August 2023 2,921 6 View

How to implement map reduce algorithm on hypercube network in matlab/ python?

Map reduce framework need to be implemented on hypercube network architecture in MATLAB or Python progeamming

14 July 2023 8,136 5 View

Topology file of PubChem CID 134149698 from swissparam?

Dear researchers, I tried to obtain a topology file of a compound with PubChem CID of 134149698 from swissparam. Following is the error message. Please let me know the way to solve it. Thanking...

12 July 2023 8,281 0 View

Hi Researchers,Can we relate the earthquake and mushroom diversity or mycorrhizal diversity? If yes, How?

Can we relate the earthquake and mushroom diversity or mycorrhizal diversity? If yes, How? Thank you.

05 June 2023 2,078 2 View

Optimization using b3lyp in GAMESS?

Can anyone help me to set the input file for optimization runs in GAMESS? Any suggestions you can give. This is what I set.

07 May 2023 8,109 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,137 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,667 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,732 2 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,113 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,056 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,672 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,404 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,418 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 840 2 View