I have been recently trying to develop a method to separate the above compounds. Most methods tried were adapted from literature using RP C8 or C18 column with ACN/MeOH and buffered water at pH 2 - 7. However, the separation was not successful. My main target is methyl salicylate (MS) but interefering from others disallowed a proper quantification of MS. I knew GCMS is a more common way to separate the compounds. But I wonder if HPLC can also do the same.
Dear Choon,
I suggest you please used the o-phosphoric acid 0.1% as a buffer under methanol/water mobile phase using the mentioned column.
Aloke Purkait Thanks for the recommendation. We will try it. May I know any reason behind this? We have tried TFA too but separation is not ideal.
Dear Choon,
First of all Trifluoroacetic acid (TFA) is a corrosive material, I'm not sure for use of it in C-18 Column.
Be noted, earlier we used o-phosphoric acid/formic acid/acetic acid as a buffer for the quantification of bioactive materials like methyl salicylate, camphor, thymol, and menthol using C-18 column.
Thanks
Please look at the following below links which may help you in your analysis:
https://absb.journals.ekb.eg/article_7204_62beec4484059a1937b7d6f4c6eee504.pdf
https://www.shodex.com/en/dc/04/02/08.html#!
https://gmpua.com/QC/ChromatAnalys.pdf
https://www.barascientific.com/article/ShimadzuMagazine/pdf-databook/Pharma%20App%20Data%20Book%20C190E075.pdf
Thanks
Isam Eldin Hussein Elgailani thanks for the recommendations. Will look through.
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