Good morning,

I am currently doing some standards for my HPLC. My interest compound is butyric acid and I am injecting it in four different concentrations in order to build the standard and the relative regression analysis (each concentration is done in triplicate). Now, for 1M, 0.5M and 0.05M I got consistent results in terms of area values, with a low deviation. But for my 0.1M sample, the sd of the area values is very high, therefore I'm not sure of the results.

Why this area values can be so high and different between each other?

Thanks,

Maurizio

Similar questions and discussions