Maurizio Cuomo

4 Questions 7 Answers 0 Followers

Questions related from Maurizio Cuomo

How to create a standard curve of enzyme concentration and relative absorbance?

Hello, during an experiment I found myself in needing a standard curve of a kit for enzyme activity screening. The kit has a certain amount of substrate (cellulose) and I prepared different...

02 February 2020 2,653 3 View

HPLC VFA analysis - why do I get so different peaks for very similar samples?

Hello, I am currently working on building standard curves for VFAs with HPLC. I've managed to get consistent results with formic, acetic, propionic and butyric acid, where the bigger the acid, the...

09 September 2019 4,725 5 View

HPLC analysis - why do I have different area values for the same sample?

Good morning, I am currently doing some standards for my HPLC. My interest compound is butyric acid and I am injecting it in four different concentrations in order to build the standard and the...

08 August 2019 3,395 6 View

HPLC analysis for VFA - how to correctly do an HPLC run?

Hello everyone, I've been using an HPLC for VFA detection but I have little experience. I'm specifically looking for acetate, propionate, butyrrate, valerate, etc. . I'm working with a c18 column...

05 May 2019 7,737 6 View