I have actinomycetes extraction and I need to detected by HPLC, so I need the protocol of hplc RP-8 chromatographic column
Please look at the following below links which may help you in your analysis:
https://pdfs.semanticscholar.org/25c4/45cdf4ab73602ef7c7232d31a2a4694baa11.pdf
http://pu.edu.pk/images/journal/zology/PDF-FILES/10-33_2_18.pdf
Thanks
Do you know which compound in the extract is of interest?
Is your goal to quantify a metabolite, or purify it?
Why did you choose that particular column?
Jack Silver
- No i don't know.
- Yes quantify a metabolite, I will purify it later, if I find something special.
- I took the protocol that I working on it from a research paper and they mentioned it and worked on it, but didn't explain it.
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